LOCUS       sequence-63275-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ914
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(423..447)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      452..775
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(779..799)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(807..824)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(807..823)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aatgcccggg ggatcggtgg agctccaccg gtggcggccg ctctagaact agtccaccaa
       61 agccatctgc caagggagcc aagaaggccg ccaagaccgt cgttgccaag ccaaaggacg
      121 gaaagaagag acgtcatgcc cgcaaggaat cgtactccgt ctacatctac cgtgttctca
      181 agcaagttca cccagacacc ggagtctcct ccaaggccat gtctatcatg aactccttcg
      241 tcaacgatgt attcgaacgc atcgcttcgg aagcttcccg tcttgctcat tacaacaaac
      301 gctcaacgat ctcatcccgc gaaattcaaa ccgctgtccg tttgattctc ccaggagaac
      361 ttgccaagca cgccgtgtct gagggaacca aggccgtcac caagtacact tccagcaagt
      421 aaacccagct ttcttgtaca aagtgggata aatgcaaaat cctttcaagc attcccttct
      481 tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat ttgctttttt
      541 aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa
      601 tgctatcaaa atgtgattct gtctgatgta ctttcacaat ctctcttcaa ttccattttg
      661 aagtgcttta aacccgaaag gttgagaaaa atgcgagcgc tcaaatattt gtattgtgtt
      721 cgttgagtga cccaacaaaa agaggaaact ttattgtgcc gccaagaaaa aagtctcaca
      781 actttattat acatagttga taattcactg gccgtcgttt tacaggccgg atgggccatg
      841 gcaccttaac atttctaaat tttagttt
//
