LOCUS       sequence-63277-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1208
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..333)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    338..362
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(364..435)
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
     primer_bind     complement(528..551)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(726..745)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aagttgtgag acttttttct tggcggcaca ataaagtttc ctctttttgt tgggtcactc
       61 aacgaacaca atacaaatat ttgagcgctc gcatttttct caacctttcg ggtttaaagc
      121 acttcaaaat ggaattgaag agagattgtg aaagtacatc agacagaatc acattttgat
      181 agcattcact tcactcagat gcaaactttt cagtgactat aaaaagtcat aaaataataa
      241 cattaaaaaa gcaaataaat tagcgagaga attttttgac aaaaagaaag aagagtgata
      301 gagaagaagg gaatgcttga aaggattttg catttatccc actttgtaca agaaagctgg
      361 gttgttctcg acctccttgg cgagcttctt ggcgttctcg gagagcttgg ttgggttggc
      421 cttacgacga cgggaagctg ggtacttgta cagctcgtcc atgccgtaca ggaacaggtg
      481 gtggcggccc tcggagcgct cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt
      541 gatgtccagc ttggtgtcca cgtagtagta gccgggcagt tgcacgggct tcttggccat
      601 gtagatggtc ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca gggcctggtg
      661 gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg cctcccagcc
      721 catggtcttc ttctgcatta cggggccgtc gggggggaag ttggtgccgc gcatcttcac
      781 cttgtagatc agcgtgccgt cctgcaggga ggagtcctgg gtcacggtca ccagaccgcc
      841 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcttgta
      901 atcggggatg tcggcggggt gcttcacgta cgccttggag ccgtacatga actgggggga
      961 caggatgtc
//
