LOCUS       sequence-63282-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Korendovych IV, Kulp DW, Wu Y, Cheng H, Roder H, DeGrado WF
  TITLE     Design of a switchable eliminase.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6823-7. Epub 2011 Apr 
            11.
  PUBMED    21482808
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-04-26"; volume: "108(17)"; pages: 
            "6823-7. Epub 2011 Apr 11"
COMMENT     Sequence Label: pEXP5-NT-AlleyCat
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(245..265)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(278..295)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             314..336
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(367..386)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(368..386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     745..764
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(844..861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tagcagccgg atcacccttt cacttcgctg tcatcatttg tacaaactct tcatagttta
       61 cttgaccatc accatcaatg tctgcttccc taatcatttc atcaacttct tcatctgtta
      121 gcttctcccc aagatttgtc atcacatgac gaagttctgc agcactaatg taaccattac
      181 catccttgtc ttccacacgg aacgcttctc taatttcttc ttcgctatct gtatctttca
      241 taagggactg aaaatacagg ttttcgccgc tgctaccatg atgatgatga tgatgagaac
      301 cagacatggt atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg
      361 tggtctccct atagtgagtc gtattaattt cgcgggatcg agatctcggg cagcgttggg
      421 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
      481 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
      541 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
      601 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
      661 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg tagcgatagc
      721 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccatt
      781 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
      841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      901 ctcaaaggcg gtaat
//
