LOCUS       sequence-63283-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a genetically-encoded sensor for lactate concentration, transport 
            and metabolic flux.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   San Martin A, Ceballo S, Ruminot I, Lerchundi R, Frommer WB, Barros 
            LF
  TITLE     A Genetically Encoded FRET Lactate Sensor and Its Use To Detect the 
            Warburg Effect in Single Cancer Cells.
  JOURNAL   PLoS One. 2013;8(2):e57712. doi: 10.1371/journal.pone.0057712. Epub 
            2013 Feb 26.
  PUBMED    23469056
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(2):e57712. doi"; pages: " 
            10.1371/journal.pone.0057712. Epub 2013 Feb 26"
COMMENT     Sequence Label: Laconic/pcDNA3.1(-)
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(27..48)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(183..695,696..698,699..701))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     376..395
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     635..656
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
       61 tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc gggcagcagc
      121 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgccgccg
      181 tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg
      241 gtgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
      301 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
      361 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
      421 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
      481 cgggcgaagc actgcaggcc gtagcccagg gtggtcacga gggtgggcca gggcacgggc
      541 agcttgccgg tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc atcgccctcg
      601 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
      661 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtagatct cttaagtgcg
      721 tttttctccc tcgaatgctc attatgctca ccgggcaggc gggtaatccg tgcgtggcga
      781 gcctgatctt catcgaatc
//
