LOCUS       sequence-63284-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a genetically-encoded sensor for lactate concentration, transport 
            and metabolic flux.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   San Martin A, Ceballo S, Ruminot I, Lerchundi R, Frommer WB, Barros 
            LF
  TITLE     A Genetically Encoded FRET Lactate Sensor and Its Use To Detect the 
            Warburg Effect in Single Cancer Cells.
  JOURNAL   PLoS One. 2013;8(2):e57712. doi: 10.1371/journal.pone.0057712. Epub 
            2013 Feb 26.
  PUBMED    23469056
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(2):e57712. doi"; pages: " 
            10.1371/journal.pone.0057712. Epub 2013 Feb 26"
COMMENT     Sequence Label: Laconic/pcDNA3.1(-)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             154..861
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
       61 ggctagcgtt taaacgggcc ctctagactc gagcggccgc cactgtgctg gatatctgca
      121 gaattccacc acactggact agtggatccg aacatggtga gcaagggcga ggagaccaca
      181 atgggcgtaa tcaagcccga catgaagatc aagctgaaga tggagggcaa cgtgaatggc
      241 cacgccttcg tgatcgaggg cgagggcgag ggcaagccct acgacggcac caacaccatc
      301 aacctggagg tgaaggaggg agcccccctg cccttctcct acgacattct gaccaccgcg
      361 ttcgcctacg gcaacagggc cttcaccaag taccccgacg acatccccaa ctacttcaag
      421 cagtccttcc ccgagggcta ctcttgggag cgcaccatga ccttcgagga caagggcatc
      481 gtgaaggtga agtccgacat ctccatggag gaggactcct tcatctacga gatacacctc
      541 aagggcgaga acttcccccc caacggcccc gtgatgcaga agaagaccac cggctgggac
      601 gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga agggcgacgt caagcacaag
      661 ctgctgctgg agggcggcgg ccaccaccgc gttgacttca agaccatcta cagggccaag
      721 aaggcggtga agctgcccga ctatcacttt gtggaccacc gcatcgagat cctgaaccac
      781 gacaaggact acaacaaggt gaccgtttac gagagcgccg tggcccgcaa ctccaccgac
      841 ggcatggacg agctgtacaa gagatctggt
//
