LOCUS       sequence-63296-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-XOPS-tdTOM
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(82..105)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(280..299)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     549..567
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      697..706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(713..727)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 cggtgctgcc ggtgccatgc cccaggaaca ggtggtggcg gccctcggag cgctcgtact
       61 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggtg tccacgtagt
      121 agtagccggg cagttgcacg ggcttcttgg ccatgtagat ggtcttgaac tccaccaggt
      181 agtggccgcc gtccttcagc ttcagggcct ggtggatctc gcccttcagc acgccgtcgc
      241 gggggtacag gcgctcggtg gaggcctccc agcccatggt cttcttctgc attacggggc
      301 cgtcgggggg gaagttggtg ccgcgcatct tcaccttgta gatcagcgtg ccgtcctgca
      361 gggaggagtc ctgggtcacg gtcaccagac cgccgtcctc gaagttcatc acgcgctccc
      421 acttgaagcc ctcggggaag gacagcttct tgtaatcggg gatgtcggcg gggtgcttca
      481 cgtacgcctt ggagccgtac atgaactggg gggacaggat gtcccaggcg aagggcaggg
      541 ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc
      601 cctcgccctc gatctcgaac tcgtggccgt tcatggagcc ctccatgcgc accttgaagc
      661 gcatgaactc tttgatgacc tcctcgccct tgctcaccat ggtggcggga tccttatcgt
      721 catcgtcgta caggtttaaa ctatcgatcc cgggtcgaac gctagcctcg agatccctag
      781 aagcctgtga tcccaactga agctgttcta cagtatgacc cactgtcccc tcgtcctttt
//
