LOCUS       sequence-63297-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Verrier JD, Madorsky I, Coggin WE, Geesey M, Hochman M, Walling E, 
            Daroszewski D, Eccles KS, Ludlow R, Semple-Rowland SL
  TITLE     Bicistronic lentiviruses containing a viral 2A cleavage sequence 
            reliably co-express two proteins and restore vision to an animal 
            model of LCA1.
  JOURNAL   PLoS One. 2011;6(5):e20553. doi: 10.1371/journal.pone.0020553. Epub 
            2011 May 27.
  PUBMED    21647387
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5):e20553. doi"; pages: " 
            10.1371/journal.pone.0020553. Epub 2011 May 27"
COMMENT     Sequence Label: pFIN-EF1a-GFP:2A:CHER(HA)-WPRE
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..54)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(55..762)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(330..349)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     599..617
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(763..819)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aatccagagg ttgattatac tagtttaagc gtagtctggg acgtcgtatg ggtacttgta
       61 cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttccacgat
      121 ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt aggcgccggg
      181 cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc
      241 gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct cggggtacat
      301 ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc cgtcggaggg
      361 gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc
      421 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
      481 ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt
      541 ggagccgtac atgaactgag gggacaggat gtcccaggcg aagggcaggg ggccaccctt
      601 ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc
      661 gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaactc
      721 cttgatgatg gccatgttat cctcctcgcc cttgctcacc ataggaccgg ggttttcttc
      781 cacgtctcct gcttgcttta acagagagaa gttcgtggct c
//
