LOCUS       sequence-63308-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   White EA, Kramer RE, Tan MJ, Hayes SD, Harper JW, Howley PM
  TITLE     Comprehensive analysis of host cellular interactions with human 
            papillomavirus E6 proteins identifies new E6 binding partners and 
            reflects viral diversity.
  JOURNAL   J Virol. 2012 Dec;86(24):13174-86. doi: 10.1128/JVI.02172-12. Epub 
            2012 Sep 26.
  PUBMED    23015706
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02172-12"; journalName: "J Virol"; date: "2012-12"; 
            volume: "86"; issue: "24"; pages: "13174-86"
COMMENT     Sequence Label: p6722 MSCV-IP N FlagHA 16E6 I128T
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             52..75
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             88..114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    130..154
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(608..632)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcttttccgc cctcctcctt ctctggcgcc ggaattagat ctgccaccat ggactacaag
       61 gatgacgatg acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga
      121 ggttatcaaa caagtttgta caaaaaagtt ggctttcagg acccacagga gcgacccaga
      181 aagttaccac agttatgcac agagctgcaa acaactatac atgatataat attagaatgt
      241 gtgtactgca agcaacagtt actgcgacgt gaggtatatg actttgcttt tcgggattta
      301 tgtatagtat atagagatgg gaatccatat gctgtatgtg ataaatgttt aaagttttat
      361 tctaaaatta gtgagtatag acattattgt tatagtgtgt atggaacaac attagaacag
      421 caatacaaca aaccgttgtg tgatttgtta attaggtgta ttaactgtca aaagccactg
      481 tgtcctgaag aaaagcaaag acatctggac aaaaagcaaa gattccataa taccaggggt
      541 cggtggaccg gtcgatgtat gtcttgttgc agatcatcaa gaacacgtag agaaacccag
      601 ctgtaatacc caactttctt gtacaaagtg gttcgataac gaattccgcc cccccc
//
