LOCUS       sequence-63315-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-mIRBP156-CHER-RK-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(117..131)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     misc_feature    443..499
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    558..675
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggtggc gggatcctta
      121 tcgtcatcgt cgtacaggtt taaactatcg atcccgggtc gaacgctagc ggcgaccggt
      181 ggatcccaga gccttggctc ctggataaga gctccgtgtg tacaaggctg tggtcatgca
      241 gctgcctctc tcccttgccc ttcttagcag gcagacagaa ggtctggggc tcaatgtggg
      301 agttagatgt gaggcttcca gctctgctaa gcctttaatc ctgtctaatt caagcacatg
      361 agctggactc tgaagaagag gccagggtgg tttgacccgg aaggaacatt gcatctcatc
      421 ttgctgagcc atgtctgtgt agggatcccg ggcccgcggt accgtcgact gcagaattcg
      481 aagcttgagc tcgagatctg agtccggtag cgcgcggccg cccaaagtgg atctctgctg
      541 tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt
      601 tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc
      661 ccttttcttt taaaattgtg gatgaatact gccatttgtg aattccggcc gcaagcccat
      721 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
      781 aaggagtgta ttaagcttgt gtaattgt
//
