LOCUS       sequence-63323-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Verrier JD, Madorsky I, Coggin WE, Geesey M, Hochman M, Walling E, 
            Daroszewski D, Eccles KS, Ludlow R, Semple-Rowland SL
  TITLE     Bicistronic lentiviruses containing a viral 2A cleavage sequence 
            reliably co-express two proteins and restore vision to an animal 
            model of LCA1.
  JOURNAL   PLoS One. 2011;6(5):e20553. doi: 10.1371/journal.pone.0020553. Epub 
            2011 May 27.
  PUBMED    21647387
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5):e20553. doi"; pages: " 
            10.1371/journal.pone.0020553. Epub 2011 May 27"
COMMENT     Sequence Label: pFIN-EF1a-GFP:2A:CHER(HA)-WPRE
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..730
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gccgctagcc gccaccatga gcaagggcga ggaactgttc actggcgtgg tcccaattct
       61 cgtggaactg gatggcgatg tgaatgggca caaattttct gtcagcggag agggtgaagg
      121 tgatgccaca tacggaaagc tcaccctgaa attcatctgc accactggaa agctccctgt
      181 gccatggcca acactggtca ctaccctgac ctatggcgtg cagtgctttt ccagataccc
      241 agaccatatg aagcagcatg actttttcaa gagcgccatg cccgagggct atgtgcagga
      301 gagaaccatc tttttcaaag atgacgggaa ctacaagacc cgcgctgaag tcaagttcga
      361 aggtgacacc ctggtgaata gaatcgagct gaagggcatt gactttaagg aggatggaaa
      421 cattctcggc cacaagctgg aatacaacta taactcccac aatgtgtaca tcatggccga
      481 caagcaaaag aatggcatca aggtcaactt caagatcaga cacaacattg aggatggatc
      541 cgtgcagctg gccgaccatt atcaacagaa cactccaatc ggcgacggcc ctgtgctcct
      601 cccagacaac cattacctgt ccacccagtc tgccctgtct aaagatccca acgaaaagag
      661 agaccacatg gtcctgctgg agtttgtgac cgctgctggg atcacacatg gcatggacga
      721 gctgtacaag gaattcccgg gtcgactcgg atcctccgga gccacgaact tctctctgtt
      781 aaagcaagca gg
//
