LOCUS       sequence-63340-e        1200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_hcRED
FEATURES             Location/Qualifiers
     source          1..1200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..61
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             111..794
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(796..820)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             822..863
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(958..978)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     gap             1101..1113
                     /estimated_length=13
ORIGIN
        1 cnnnnnnntg acgcnnatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
       61 tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agttggcacc atggtgagcg
      121 gcctgctgaa ggagagtatg cgcatcaaga tgtacatgga gggcaccgtg aacggccact
      181 acttcaagtg cgagggcgag ggcgacggca accccttcgc cggcacccag agcatgagaa
      241 tccacgtgac cgagggcgcc cccctgccct tcgccttcga catcctggcc ccctgctgcg
      301 agtacggcag caggaccttc gtgcaccaca ccgccgagat ccccgacttc ttcaagcaga
      361 gcttccccga gggcttcacc tgggagagaa ccaccaccta cgaggacggc ggcatcctga
      421 ccgcccacca ggacaccagc ctggagggca actgcctgat ctacaaggtg aaggtgcacg
      481 gcaccaactt ccccgccgac ggccccgtga tgaagaacaa gagcggcggc tgggagccca
      541 gcaccgaggt ggtgtacccc gagaacggcg tgctgtgcgg ccggaacgtg atggccctga
      601 aggtgggcga ccggcacctg atctgccacc actacaccag ctaccggagc aagaaggccg
      661 tgcgcgccct gaccatgccc ggcttccact tcaccgacat ccggctccag atgctgcgga
      721 agaagaagga cgagtacttc gagctgtacg aggccagcgt ggcccggtac agcgacctgc
      781 ccgagaaggc caacttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc
      841 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
      901 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggnattc ntaactatgt
      961 tgctcctttt acgctatgtg gatangctgc tttnatgnnt ttgtatcatg ctantgcttc
     1021 ccgtatggnt ttcatttttc ncnnnnnnnt aaancnnnnn ctgtcnnntt atnnannann
     1081 nnnnnccnnn nncngnnanc nnnnnnnnnn nnnannnnnn ttnnctnann caacncnann
     1141 gnnnngnnnn tgncnnnnnn ncannnnnnn ngnnnnnnnn ntnnncnncn nnnnnnnncc
//
