LOCUS       sequence-63347-e        1212 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_TLOC1 (220aa)
FEATURES             Location/Qualifiers
     source          1..1212
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     266..286
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(381..422)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    424..448
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     gap             1157..1173
                     /estimated_length=17
     gap             1198..1209
                     /estimated_length=12
ORIGIN
        1 agcgggcagg caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt
       61 cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa
      121 cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag
      181 caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata
      241 caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc
      301 agtcaatctt tcacaaattt tgtaatccag aggttgattg tcgacttaac gcgccaccgg
      361 ttagcgctag ctcattacta cgtagaatcg agaccgagga gagggttagg gataggctta
      421 ccaaccactt tgtacaagaa agttgggtat gatttttcat gtgaagattt aggtgtttct
      481 ccatcatttt cctcttcctc atcctcttca caatccccat ctgtttgctg ttccagttcc
      541 tcttttgtta tcatttcaaa atcatttcca tccagaaaaa cctgatcatc atgtggctca
      601 agtttgaatt ttttcttagt ttccttcttt ttaggagttc ctggagtttc ctcctttttg
      661 gattcttcct tttcaccatc tttttttttc tctttttctt ttnntgtctt ctcatccttt
      721 atattttctt tcttgctctt tttatcttct tcttttccac tttcagcttt tcctttatct
      781 ttttctttct ttatgtcttt atcatatttc attttcatta cttttagggc tcggtgaaaa
      841 aactgcttct ttaaaagcct gttgcagtag tcaaccacag actccctggt tgtaaataaa
      901 gcttcctctc ctttcttggn cctttgccca ctttgaatcc aaaagacagt ccactgcttt
      961 tgaagcaata aaataatcaa cccggtgacc catcatattg gngggacttt gttggacagt
     1021 tgaatcnana tacttggnna cancntnctc ttctttanat ggnncancna nnttccngga
     1081 tccgcttctt gngnnnnncn ncntncngnc anncnntttt tngtacnann nnnatccnna
     1141 nnnnnnnncn nannncnnnn nnnnnnnnnn nnnccnnnna acnncnnncn nnnnngcnnn
     1201 nnnnnnnnng na
//
