LOCUS       sequence-63354-e        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_LUC
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..53
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    76..100
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(169..187)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     gap             1101..1111
                     /estimated_length=11
ORIGIN
        1 ttgacgcnnt gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
       61 taactgtcgg gatcaacaag tttgtacaaa aaagttggca ccatggaaga cgccaaaaac
      121 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg
      181 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
      241 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct
      301 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
      361 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
      421 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg
      481 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc
      541 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
      601 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc
      661 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa
      721 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt
      781 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
      841 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
      901 agatttgaag aananctgtt tctgaggagc cttcnnntta cnagattcaa agtgcgctgc
      961 tggtgccanc ctattctcnt cttcncaaag cactctgatt ganaanncga tttatctant
     1021 tacacgaaat tgntnnnnnn nnctccccnc nnntannntc ggggnnnnnn cnnnnnnnnc
     1081 tncngnntca gcnngnnatg nnnnnnnnnn ntnnnnagct attcngatnn nccnannggg
     1141 nnnnnannnn gncnnnnnc
//
