LOCUS       sequence-63355-e        1225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaC27
FEATURES             Location/Qualifiers
     source          1..1225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..11
                     /estimated_length=10
     primer_bind     278..298
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(393..434)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    436..460
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     gap             1174..1185
                     /estimated_length=12
ORIGIN
        1 cnnnnnnnnn nagcgggcan ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
       61 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
      121 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
      181 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
      241 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
      301 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tgtcgactta
      361 acgcgccacc ggttagcgct agctcattac tacgtagaat cgagaccgag gagagggtta
      421 gggataggct taccaaccac tttgtacaag aaagttgggt attccagttc ctcttttgtt
      481 atcatttcaa aatcatttcc atccagaaaa acctgatcat catgtggctc aagtttgaat
      541 tttttcttag tttccttctt tttaggagtt cctggagttt cctccttttt ggattcttcc
      601 ttttcaccat cttttttttt ctctttttct ttttttgtct tctcatcctt tatattttct
      661 ttcttgctct ttttatcttc ttcttttcca ctttcagctt ttcctttatc tttttctttc
      721 tttatgtctt tatcatattt cattttcatt acttttaggg ctcggtgaaa aaactgcttc
      781 tttaaaagcc tgttgcagta gtcaaccaca gactccctgg ttgtaaataa agcttcctct
      841 cctttcttgg cctttgccca ctttgaatcc aaaagacagt ccactgcttt tgaagcaata
      901 aaataatcaa cccggtgacc catcatattg gtggactttg ttggacagtt gaatcgaaga
      961 tacttggncn ncagcccttc tcttctttag atgggttcac caactttcct ggatccgctt
     1021 cntgtgtctc ctgcgttcnc catgccaact tttttngtac nancttgtna tcccgacnnt
     1081 tanccagann gctctgntnn ntngacntcc cancgnacac gcnncnnnnt ngcgnnnang
     1141 gggncnnnnn nntncnannt tttnnnaann tccnnnnnnn nnnnnannnn nnccnnnacg
     1201 tcangnnnng nnnnnnntnn nnnnc
//
