LOCUS       sequence-63357-e        1180 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN81
FEATURES             Location/Qualifiers
     source          1..1180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             13..23
                     /estimated_length=11
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(533..557)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             559..600
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(695..715)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cnccccnntt tgnnnnnnnn nnnggcggta ggngngtacg gngggaggtc tatataagca
       61 gagctctctg gctnactgtc gggatcaaca agtttgtaca aaaaagttgg catgtgcaac
      121 aggcttttaa agaagcagtt ttttcaccga gccctaaaag tanngaaaat gaaatatgat
      181 aaagacataa agaaagaaaa agataaagga aaagctgaaa gtggaaaaga agaagataaa
      241 aagagcaaga aagaaaatat aaaggatgag aagacaaaaa aagaaaaaga gaaaaaaaaa
      301 gatggtgaaa aggaagaatc caaaaaggag gaaactccag gaactcctaa aaagaaggaa
      361 actaagaaaa aattcaaact tgagccacat gatgatcagg tttttctgga tggaaatgat
      421 tttgaaatga taacaaaaga ggaactggaa cagcaaacag atggggattg tgaagaggat
      481 gaggaagagg aaaatgatgg agaaacacct aaatcttcac atgaaaaatc atacccaact
      541 ttcttgtaca aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg
      601 tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggattaca
      661 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
      721 acgctgcttt aatgcctttt gtatcatgct attgcttccc gtatggcttt catttttctc
      781 ctccttgtat aaatcctgnt tgctgtctct tttatgagga gtttgtggcc cgttgtcang
      841 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc cccactggtt ggggcattgc
      901 caccacctgt cagctccttt cccgggactt tcgctttccc cctccctatt gccacggcgg
      961 aactcatcnc cgcctngcct nncccgctgc tggacagggg ctcggctgtt gggnactgaa
     1021 caattccgng gngttgtccg ggaaaatcat cgtcctttcc ctnngnnngc tccgccngnn
     1081 nnngccnccn nnnntcnnnn nnngnacgtt ccttnctgnn tacgttccnt nnngtccnnn
     1141 aannnannng ancnnnnnct tnnncnngng ngncngnnnt
//
