LOCUS       sequence-63358-e        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN81
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     271..291
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(386..427)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    429..453
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(874..898)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(921..972)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(950..970)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cnnnagcgnn nnnngcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
       61 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
      121 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
      181 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
      241 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
      301 ataccagtca atctttcaca aattttgtaa tccagaggtt gattgtcgac ttaacgcgcc
      361 accggttagc gctagctcat tactacgtag aatcgagacc gaggagaggg ttagggatag
      421 gcttaccaac cactttgtac aagaaagttg ggtatgattt ttcatgtgaa gatttaggtg
      481 tttctccatc attttcctct tcctcatcct cttcacaatc cccatctgtt tgctgttcca
      541 gttcctcttt tgttatcatt tcaaaatcat ttccatccag aaaaacctga tcatcatgtg
      601 gctcaagttt gaattttttc ttagtttcct tctttttagg agttcctgga gtttcctcct
      661 ttttggattc ttccttttca ccatcttttt ttttctcttt ttcttttttt gtcttctcat
      721 cctttatatt ttctttcttg ctctttttat cttcttcttt tccactttca gcttttcctt
      781 tatctttttc tttctttatg tctttatcat atttcatttt cattactttt agggctcggt
      841 gaaaaaactg cttctttaaa agcctgttgc acatgccaac ttttttgtac aaacttgttg
      901 atcccgacag ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg
      961 cccatttgcg tcaatggggc gganttgtta cnacattttn gnaaagtccc gttnattttg
     1021 gnncnaaaca actcccattg acgtcanggg nggnnacntn gnaatccccg ngantcnaac
     1081 nctatccncn cccnnttgat nnnctnccaa aaccnnnnnc nncccnnggn nannnnnnnn
     1141 ntgannnnaa nanc
//
