LOCUS       sequence-63361-e        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN149
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..14
                     /estimated_length=13
     promoter        20..63
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    86..110
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(327..351)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             353..394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    436..1024
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(489..509)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(907..918)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cnnnnnnnnn nnnngcannt gggcggtagg cgtgtacggt gggaggtcta tataagcaga
       61 gctctctggc taantgtcgg gatcaacaag tttgtacaaa aaagttggca tgtccaaaaa
      121 ggaggaaact ccaggaactc ctaaaaagaa ggaaactaag aaaaaattca aacttgagcc
      181 acatgatgat caggtttttc tggatggaaa tgattttgaa atgataacaa aagaggaact
      241 ggaacagcaa acagatgggg attgtgaaga ggatgaggaa gaggaaaatg atggagaaac
      301 acctaaatct tcacatgaaa aatcataccc aactttcttg tacaaagtgg ttggtaagcc
      361 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg
      421 gcgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
      481 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
      541 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
      601 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
      661 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
      721 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
      781 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
      841 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
      901 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
      961 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     1021 ccgcgtcgac tttaagacaa tgacttanna ggcagctgta natccttagc cantttttaa
     1081 aanaaaaagg ggggactggn aggnntaatt tnnnctnncc annnaaanan naana
//
