LOCUS       sequence-63366-e        1198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pLEX_970_puro_DEST_V5_BFP
FEATURES             Location/Qualifiers
     source          1..1198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        15..53
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    76..100
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(103..105,106..108,109..819)
                     /codon_start=1
                     /product="blue variant of GFP"
                     /label=BFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDXLYK"
     primer_bind     complement(148..169)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(409..428)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     756..777
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(821..920)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(937..956)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(938..956)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gap             1055..1065
                     /estimated_length=11
     gap             1090..1102
                     /estimated_length=13
     gap             1125..1137
                     /estimated_length=13
     gap             1164..1175
                     /estimated_length=12
     gap             1184..1195
                     /estimated_length=12
ORIGIN
        1 ttgacgcnnn gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
       61 taactgtcgg gatcaacaag tttgtacaaa aaagttggca ccatggtgag caagggcgag
      121 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      181 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      241 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgagc
      301 cacggcgtcc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      361 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      421 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      481 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttc
      541 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggcgaacttc
      601 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      661 acccccatcg gcgacggccc cgtgctgctg cccgacagcc actacctgag cacccagtcc
      721 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      781 gccgccggga tcactctcgg catggacgan ctgtacaagt tgccaacttt cttgtacaaa
      841 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
      901 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
      961 gncatagctn nttcctggna gctcnnggcc cgngtctcaa aatctctgat gttacnttgc
     1021 acagataaaa atntatcatc atgccnnnnt agacnnnnnn nnnnnaaatn nnncnnnntn
     1081 ncngctnccn nnnnnnnnnn nncnnncgnn naacgnatnn nnncnnnnnn nnnnnnnanc
     1141 nnnncnnnnn nancnnnnnn cagnnnnnnn nnnnntnnnn nncnnnnnnn nnnnnaac
//
