LOCUS       sequence-63367-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Ramaswamy S, Nakamura N, Vazquez F, Batt DB, Perera S, Roberts TM, 
            Sellers WR
  TITLE     Regulation of G1 progression by the PTEN tumor suppressor protein is
            linked to inhibition of the phosphatidylinositol 3-kinase/Akt 
            pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5.
  PUBMED    10051603
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Mar 2. 96(5):2110-5."
COMMENT     Sequence Label: 903 pLNCX HA Akt1
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             139..165
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgcggcccca agctggaaga
      121 tccgccgcca ccatgggtta cccatacgac gtgccagatt acgccagcga cgtggctatt
      181 gtgaaggagg gttggctgca caaacgaggg gagtacatca agacctggcg gccacgctac
      241 ttcctcctca agaatgatgg caccttcatt ggctacaagg agcggccgca ggatgtggac
      301 caacgtgagg ctcccctcaa caacttctct gtggcgcagt gccagctgat gaagacggag
      361 cggccccggc ccaacacctt catcatccgc tgcctgcagt ggaccactgt catcgaacgc
      421 accttccatg tggagactcc tgaggagcgg gaggagtgga caaccgccat ccagactgtg
      481 gctgacggcc tcaagaagca ggaggaggag gagatggact tccggtcggg ctcacccagt
      541 gacaactcag gggctgaaga gatggaggtg tccctggcca agcccaagca ccgcgtgacc
      601 atgaacgagt ttgagtacct gaagctgctg ggcaagggca ctttcggcaa ggtgatcctg
      661 gtgaaggaga aggccacagg ccgctactac gccatgaaga tcctcaagaa ggaagtcatc
      721 gtagccaagg acgaagtggc ccacacactc accgagaacc gcgtcctgca gaactccagg
      781 caccccttcc tcacagccct gaagtactct ttccagaccc acgaccgcct ctgctttgtc
      841 atggagtacg ccaacggggg cgagctgttc ttccacctgt cccgggagcg tgtgttctcc
      901 gaggaccgg
//
