LOCUS       sequence-63370-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Willcox BE, Thomas LM, Bjorkman PJ
  TITLE     Crystal structure of HLA-A2 bound to LIR-1, a host and viral major 
            histocompatibility complex receptor.
  JOURNAL   Nat Immunol. 2003 Sep . 4(9):913-9.
  PUBMED    12897781
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Immunol. 2003 Sep . 4(9):913-9."
COMMENT     Sequence Label: pET23a-LIR-1 D1-D2
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             655..672
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(725..743)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      739..786
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(910..929)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttccctcta gaaataattt tgtttaactt taagaaggag atatacatat ggggcacctc
       61 cccaagccca ccctctgggc tgaaccaggc tctgtgatca cccaggggag tcctgtgacc
      121 ctcaggtgtc aggggggcca ggagacccag gagtaccgtc tatatagaga aaagaaaaca
      181 gcaccctgga ttacacggat cccacaggag cttgtgaaga agggccagtt ccccatccca
      241 tccatcacct gggaacatgc agggcggtat cgctgttact atggtagcga cactgcaggc
      301 cgctcagaga gcagtgaccc cctggagctg gtggtgacag gagcctacat caaacccacc
      361 ctctcagccc agcccagccc cgtggtgaac tcaggaggga atgtaaccct ccagtgtgac
      421 tcacaggtgg catttgatgg cttcattctg tgtaaggaag gagaagatga acacccacaa
      481 tgcctgaact cccagcccca tgcccgtggg tcgtcccgcg ccatcttctc cgtgggcccc
      541 gtgagcccga gtcgcaggtg gtggtacagg tgctatgctt atgactcgaa ctctccctat
      601 gagtggtctc tacccagtga tctcctggag ctcctggtcc tatcatgact cgagcaccac
      661 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      721 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      781 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      841 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      901 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      961 gtcaagctct aaatcggggg ctccctttag ggttcc
//
