LOCUS       sequence-63374-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-G1TCbt
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(60..341)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(362..381)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     481..503
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(541..559)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        627..731
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgcaagccgc aaactttcac caatggacca gaactacctg tgaaattaat aacagacata
       61 ctccaagctg cctttgtgtg cttaatcacg tatactcacg tgctcaatag tcaccaatgc
      121 cctccctctt ggccctctcc ttttcttttt tcgaccgaat taattcttaa tcggcaaaaa
      181 aagaaaagct ccggatcaag attgtacgta aggtgacaag ctatttttca ataaagaata
      241 tcttccacta ctgccatctg gcgtcataac tgcaaagtac acatatatta cgatgctgtt
      301 ctattaaatg cttcctatat tatatatata gtaatgtcgt tgatctatgg tgcactctca
      361 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
      421 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      481 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
      541 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      601 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      721 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      781 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      841 agttgg
//
