LOCUS       sequence-63389-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M
  TITLE     Quantitative understanding of the energy transfer between 
            fluorescent proteins connected via flexible peptide linkers.
  JOURNAL   Biochemistry. 2006 Nov 7. 45(44):13183-92.
  PUBMED    17073440
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2006 Nov 7. 45(44):13183-92."
COMMENT     Sequence Label: pET28CLY1
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             28..50
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             69..86
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..113
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(117..119,120..122,123..833)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(423..442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     770..791
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tatactatgg
       61 gcagcagcca tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgg
      121 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      181 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      241 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      301 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      361 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      421 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      481 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      541 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
      601 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      661 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      721 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      781 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggaa
      841 ttcgtggtgg atccggaggt agcaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      901 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtc
//
