LOCUS       sequence-63399-e       12737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Murakami KS
  TITLE     X-ray Crystal Structure of Escherichia coli RNA Polymerase sigma70 
            Holoenzyme.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):9126-34. doi: 
            10.1074/jbc.M112.430900. Epub 2013 Feb 6.
  PUBMED    23389035
REFERENCE   2  (bases 1 to 12737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.430900"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "9126-34"
COMMENT     Sequence Label: pGEMABC
FEATURES             Location/Qualifiers
     source          1..12737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9689..9706)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9764..9782)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9778..9825
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(9924..10379)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10061..10082)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10273..10292
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(10448..10467)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10567..10589
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10627..10645)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10713..10817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10818..10886,10887..11678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11849..12437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12338..12357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12591..12608
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     12700..12719
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12700..12718
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctagagagc ttagtaccaa agagaggaca caatgcaggg ttctgtgaca gagtttctaa
       61 aaccgcgcct ggttgatatc gagcaagtga gttcgacgca cgccaaggtg acccttgagc
      121 ctttagagcg tggctttggc catactctgg gtaacgcact gcgccgtatt ctgctctcat
      181 cgatgccggg ttgcgcggtg accgaggttg agattgatgg tgtactacat gagtacagca
      241 ccaaagaagg cgttcaggaa gatatcctgg aaatcctgct caacctgaaa gggctggcgg
      301 tgagagttca gggcaaagat gaagttattc ttaccttgaa taaatctggc attggccctg
      361 tgactgcagc cgatatcacc cacgacggtg atgtcgaaat cgtcaagccg cagcacgtga
      421 tctgccacct gaccgatgag aacgcgtcta ttagcatgcg tatcaaagtt cagcgcggtc
      481 gtggttatgt gccggcttct acccgaattc attcggaaga agatgagcgc ccaatcggcc
      541 gtctgctggt cgacgcatgc tacagccctg tggagcgtat tgcctacaat gttgaagcag
      601 cgcgtgtaga acagcgtacc gacctggaca agctggtcat cgaaatggaa accaacggca
      661 caatcgatcc tgaagaggcg attcgtcgtg cggcaaccat tctggctgaa caactggaag
      721 ctttcgttga cttacgtgat gtacgtcagc ctgaagtgaa agaagagaaa ccagagttcg
      781 atccgatcct gctgcgccct gttgacgatc tggaattgac tgtccgctct gctaactgcc
      841 ttaaagcaga agctatccac tatatcggtg atctggtaca gcgtaccgag gttgagctcc
      901 ttaaaacgcc taaccttggt aaaaaatctc ttactgagat taaagacgtg ctggcttccc
      961 gtggactgtc tctgggcatg cgcctggaaa actggccacc ggcaagcatc gctgacgagt
     1021 aaccggatcc gggccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct
     1081 tgtttctatc gacgacttaa tatactgcga caggacgtcc gttctgtgta aatcgcaatg
     1141 aaatggttta agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca
     1201 aaatagtgtt gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg
     1261 aggaacccta tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa
     1321 cgtccacaag ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa
     1381 tttatcgagc aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc
     1441 ccgattcaga gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa
     1501 ccggtgtttg acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc
     1561 gttaaactgc gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt
     1621 aaagaacaag aagtctacat gggcgaaatt ccgctcatga cagacaacgg tacctttgtt
     1681 atcaacggta ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg cgtcttcttt
     1741 gactccgaca aaggtaaaac ccactcttcg ggtaaagtgc tgtataacgc gcgtatcatc
     1801 ccttaccgtg gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt
     1861 atcgaccgtc gccgtaaact gcctgcgacc atcattctgc gcgccctgaa ctacaccaca
     1921 gagcagatcc tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagctg
     1981 cagatggaac tggtgccgga acgcctgcgt ggtgaaaccg catcttttga catcgaagct
     2041 aacggtaaag tgtacgtaga aaaaggccgc cgtatcactg cgcgccacat tcgccagctg
     2101 gaaaaagacg acgtcaaact gatcgaagtc ccggttgagt acatcgcagg taaagtggtt
     2161 gctaaagact atattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg
     2221 agcctggatc tgctggctaa gctgagccag tctggtcaca agcgtatcga aacgctgttc
     2281 accaacgatc tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac
     2341 gaccgtctga gcgcactggt agaaatctac cgcatgatgc gccctggcga gccgccgact
     2401 cgtgaagcag ctgaaagcct gttcgagaac ctgttcttct ccgaagaccg ttatgacttg
     2461 tctgcggttg gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc
     2521 ggtatcctga gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac
     2581 ggtaaaggcg aagtcgatga tatcgaccac ctcggcaacc gtcgtatccg ttccgttggc
     2641 gaaatggcgg aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag
     2701 cgtctgtctc tgggcgatct ggataccctg atgccacagg atatgatcaa cgccaagccg
     2761 atttccgcag cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggtccag
     2821 aacaacccgc tgtctgagat tacgcacaaa cgtcgtatct ccgcactcgg cccaggcggt
     2881 ctgacccgtg aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc
     2941 gtatgtccaa tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc tctgtccgtg
     3001 tacgcacaga ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt
     3061 gttgtaactg acgaaattca ctacctgtct gctatcgaag aaggcaacta cgttatcgcc
     3121 caggcgaact ccaacttgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt
     3181 agcaaaggcg aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc
     3241 cagcaggtgg tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac
     3301 cgtgcattga tgggtgcgaa catgcaacgt caggccgttc cgactctgcg cgctgataag
     3361 ccgctggttg gtactggtat ggaacgtgct gttgccgttg actccggtgt aactgcggta
     3421 gctaaacgtg gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac
     3481 gaagacgaga tgtatccggg tgaagcaggt atcgacatct acaacctgac caaatacacc
     3541 cgttctaacc agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggtt
     3601 gaacgtggcg acgtgctggc agacggtccg tccaccgacc tcggtgaact ggcgcttggt
     3661 cagaacatgc gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc
     3721 gtatccgagc gtgttgttca ggaagaccgt ttcaccacca tccacattca ggaactggcg
     3781 tgtgtgtccc gtgacaccaa gctgggtccg gaagagatca ccgctgacat cccgaacgtg
     3841 ggtgaagctg cgctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg
     3901 accggtggcg acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca
     3961 gaagaaaaac tgctgcgtgc gatcttcggt gagaaagcct ctgacgttaa agactcttct
     4021 ctgcgcgtac caaacggtgt atccggtacg gttatcgacg ttcaggtctt tactcgcgat
     4081 ggcgtagaaa aagacaaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag
     4141 aaagacctgt ctgaagaact gcagatcctc gaagcgggtc tgttcagccg tatccgtgct
     4201 gtgctggtag ccggtggcgt tgaagctgag aagctcgaca aactgccgcg cgatcgctgg
     4261 ctggagctgg gcctgacaga cgaagagaaa caaaatcagc tggaacagct ggctgagcag
     4321 tatgacgaac tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc
     4381 cagggcgacg atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa
     4441 cgccgtatcc agcctggtga caagatggca ggtcgtcacg gtaacaaggg tgtaatttct
     4501 aagatcaacc cgatcgaaga tatgccttac gatgaaaacg gtacgccggt agacatcgta
     4561 ctgaacccgc tgggcgtacc gtctcgtatg aacatcggtc agatcctcga aacccacctg
     4621 ggtatggctg cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa
     4681 gtcgcgaaac tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga cgttcgtcag
     4741 aaagttgacc tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc
     4801 aaaggtatgc caatcgcaac gccggtgttc gacggtgcga aagaagcaga aattaaagag
     4861 ctgctgaaac ttggcgacct gccgacttcc ggtcagatcc gcctgtacga tggtcgcact
     4921 ggtgaacagt tcgagcgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac
     4981 ctggtcgacg acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag
     5041 ccgctgggtg gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg
     5101 ctggaagcat acggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac
     5161 gtgaacggtc gtaccaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg
     5221 ggcatgccag aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc
     5281 gaactggaag acgagtaatt ctcgctcaaa caggtcactg ctgtcgggtt aaaacccggc
     5341 agcggattgt gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa
     5401 agcgcagact aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat
     5461 gatccgttca tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt
     5521 caaaccagaa cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga
     5581 gtgcctgtgc ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg
     5641 cgttgaagtg acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc
     5701 cccgactgcg cacatctggt tcctgaaatc gctgccgtcc cgtatcggtc tgctgctcga
     5761 tatgccgctg cgcgatatcg aacgcgtact gtactttgaa tcctatgtgg ttatcgaagg
     5821 cggtatgacc aacctggaac gtcagcagat cctgactgaa gagcagtatc tggacgcgct
     5881 ggaagagttc ggtgacgaat tcgacgcgaa gatgggggcg gaagcaatcc aggctctgct
     5941 gaagagcatg gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa
     6001 ctccgaaacc aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca
     6061 gtctggtaac aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct
     6121 gcgtccgctg gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta
     6181 tcgtcgcgtc attaaccgta acaaccgtct gaaacgtctg ctggatctgg ctgcgccgga
     6241 catcatcgta cgtaacgaaa aacgtatgct gcaggaagcg gtagacgccc tgctggataa
     6301 cggtcgtcgc ggtcgtgcga tcaccggttc taacaagcgt cctctgaaat ctttggccga
     6361 catgatcaaa ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gtgttgacta
     6421 ctccggtcgt tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc
     6481 gaagaaaatg gcactggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg
     6541 tcttgctacc accattaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg
     6601 ggatatcctg gacgaagtta tccgcgaaca cccggtactg ctgaaccgtg caccgactct
     6661 gcaccgtctg ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct
     6721 gcacccgctg gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggctgttca
     6781 cgtaccgctg acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa
     6841 catcctgtcc ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg
     6901 tctgtactac atgacccgtg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg
     6961 cccgaaagaa gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa
     7021 agtgcgtatc accgagtatg aaaaagatgc taacggtgaa ttagtagcga aaaccagcct
     7081 gaaagacacg actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta
     7141 ctccatcgtc aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta
     7201 ccgcattctc ggtctgaaac cgaccgttat ttttgcggac cagatcatgt acaccggctt
     7261 cgcctatgca gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa
     7321 gaaacacgaa atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca
     7381 gtctggtctg gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc
     7441 gaacgatcgt gtatccaaag cgatgatgga taacctgcaa actgaaaccg tgattaaccg
     7501 tgacggtcag gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg
     7561 tgcgcgtggt tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa
     7621 gccggatggc tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt
     7681 actccagtac ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa
     7741 aactgcgaac tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt
     7801 taccgaagac gattgtggta cccatgaagg tatcatgatg actccggtta tcgagggtgg
     7861 tgacgttaaa gagccgctgc gcgatcgcgt actgggtcgt gtaactgctg aagacgttct
     7921 gaagccgggt actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg
     7981 tgacctgctg gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga
     8041 caccgacttt ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat
     8101 caacaagggt gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca
     8161 gctgaccatg cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag
     8221 catccaagtg aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc
     8281 cagcggtaaa ctggttatca cttcccgtaa tactgaactg aaactgatcg acgaattcgg
     8341 tcgtactaaa gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga
     8401 acaggttgct ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac
     8461 cgaagtaagc ggttttgtac gctttactga catgatcgac ggccagacca ttacgcgtca
     8521 gaccgacgaa ctgaccggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc
     8581 aggtggtaaa gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct
     8641 gatcccaggt accgatatgc cagcgcagta cttcctgccg ggtaaagcga ttgttcagct
     8701 ggaagatggc gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg
     8761 cggtaccaag gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg
     8821 tccgaaagag ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac
     8881 caaaggtaaa cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat
     8941 gattccgaaa tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt
     9001 aatttccgac ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt
     9061 tactcgttac atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa
     9121 cgataaacac atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc
     9181 gggtagctcc gacttcctgg aaggcgaaca ggttgaatac tctcgcgtca agatcgcaaa
     9241 ccgcgaactg gaagcgaacg gcaaagtggg tgcaacttac tcccgcgatc tgctgggtat
     9301 caccaaagcg tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac
     9361 tcgcgtgctg accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga
     9421 gaacgttatc gtgggtcgtc tgatcccggc aggtaccggt tacgcgtacc accaggatcg
     9481 tatgcgtcgc cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc
     9541 atctgccagc ctggcagaac tgctgaacgc aggtctgggc ggttctgata acgagtaatc
     9601 gttaatccgc aaataacgta aaaacccgct tcggcgggtt tttttatggg gggagtttag
     9661 ggaaagagca tttgtcagaa tccggatcct atagtgtcac ctaaatccca gcttgatccg
     9721 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     9781 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     9841 atatccggat aacctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     9901 tgcgcagcct gaatggcgaa tggaaattgt aaacgttaat attttgttaa aattcgcgtt
     9961 aaatatttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
    10021 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
    10081 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
    10141 cccactacgt gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc gtaaagctct
    10201 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
    10261 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
    10321 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
    10381 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
    10441 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
    10501 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
    10561 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
    10621 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
    10681 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10741 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10801 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10861 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10921 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10981 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    11041 gtcatacact attatcccgt attgacgccg ggcaagagca actcggtcgc cgggcgcggt
    11101 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    11161 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    11221 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    11281 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    11341 agcgtgacac cacgatgcct gtagcaatgc caacaacgtt gcgcaaacta ttaactggcg
    11401 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    11461 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    11521 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    11581 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11641 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    12001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    12061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    12121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    12181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    12241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    12301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    12361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    12421 ggcggagcct atcgaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    12481 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    12541 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12601 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    12661 ttcattaatg cagcccggga gatctcgatc ccgcgaaatt aatacgactc actataggga
    12721 gaccacaacg gtttccc
//
