LOCUS       sequence-63411-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Chubiz LM, Lee MC, Delaney NF, Marx CJ
  TITLE     FREQ-Seq: a rapid, cost-effective, sequencing-based method to 
            determine allele frequencies directly from mixed populations.
  JOURNAL   PLoS One. 2012;7(10):e47959. doi: 10.1371/journal.pone.0047959. Epub
            2012 Oct 31.
  PUBMED    23118913
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e47959. doi"; pages: " 
            10.1371/journal.pone.0047959. Epub 2012 Oct 31"
COMMENT     Sequence Label: pFSBC42
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     405..421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(467..486)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     586..608
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(646..664)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      241 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      301 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa aatgatacgg cgaccaccga
      361 gatctacact ctttccctac acgacgctct tccgatctga accagtaaaa cgacggccag
      421 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
      481 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      541 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
      601 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
      661 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      721 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      781 cgctcatgag acaataaccc tgataaatgc ttcaataata ttg
//
