LOCUS       sequence-63421-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Krupka N, Strappe P, Gotz J, Ittner LM
  TITLE     Gateway-compatible lentiviral transfer vectors for ubiquitin 
            promoter driven expression of fluorescent fusion proteins.
  JOURNAL   Plasmid. 2010 May;63(3):155-60. doi: 10.1016/j.plasmid.2010.01.002. 
            Epub 2010 Feb 2.
  PUBMED    20132838
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2010.01.002"; journalName: "Plasmid"; date: 
            "2010-05"; volume: "63"; issue: "3"; pages: "155-60"
COMMENT     Sequence Label: pLVU/RED
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             373..678
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     557..576
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(719..843)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag
       61 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
      121 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
      181 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
      241 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
      301 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
      361 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
      421 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
      481 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
      541 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
      601 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
      661 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
      721 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      781 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
      841 ggttgatatc
//
