LOCUS       sequence-63424-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YAP/TAZ-responsive synthetic promoter driving luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Dupont S, Morsut L, Aragona M, Enzo E, Giulitti S, Cordenonsi M, 
            Zanconato F, Le Digabel J, Forcato M, Bicciato S, Elvassore N, 
            Piccolo S
  TITLE     Role of YAP/TAZ in mechanotransduction.
  JOURNAL   Nature. 2011 Jun 8;474(7350):179-83. doi: 10.1038/nature10137.
  PUBMED    21654799
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-06-8- 8"; volume: "474"; issue: "7350"; pages:
            "179-83"
COMMENT     Sequence Label: 8xGTIIC-luciferase
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(181..197)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(182..198)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    join(224..230,231..232,233..239)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(256..262,263..264,265..271)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(310..316,317..318,319..325)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(342..348,349..350,351..357)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     misc_feature    414..505
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(446..465)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    519..567
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(835..856)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgcca agctcttggg ggatcgtccc acggagcggt gggtgccggc ggctgtctgg
      121 gaagggctcc ttggggggca gaggctttaa ggtccccccg gcgcccaccc ccaagcttat
      181 cgataccgtc gacctcgacc tgcagatctc tagaagcttg cagtggaatg tgtggaatgt
      241 gtctcaagct tgcagtggaa tgtgtggaat gtgtctcaac ttggcactgg ccgggctaga
      301 agcttgcagt ggaatgtgtg gaatgtgtct caagcttgca gtggaatgtg tggaatgtgt
      361 ctcaacttgg cactggccgg gctagcacgc gtaagagctc ggtacctatc gatagagaaa
      421 tgttctggca cctgcacttg cactggggac agcctatttt gctagtttgt tttgtttcgt
      481 tttgttttga tggagagcgt atgttagtac tatcgattca cacaaaaaac caacacacag
      541 atgtaatgaa aataaagata ttttattgcg gccgctccaa gtacctcccg taccttaata
      601 ttacttactt atcatggtag cttgggctgg cgtaatagcg aagaggcccg caccgatcgc
      661 ccttcccaac agttgcgcag cctgaatggc gaatggcaaa ttgtaagcgt taatattttg
      721 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
      781 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
      841 tggaacaaga gtccactatt aaaagaacgt ggactccaac gtcaaaaggc gaaaaaccgt
      901 ctatcagggc gaatggccca ctacgtgaaa ccatcaccct aatcagtttt tgg
//
