LOCUS       sequence-63431-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral promoter-less Gateway destination vector, Zeo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X1 Zeo DEST (668-1)
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    43..631
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             149..160
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     558..578
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    662..786
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aattccaaac tcattactaa ccggatatct gcagaattcc aggcggggag gcggcccaaa
       61 gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca
      121 gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg
      181 tcccgcgcag aatccaggtg gcaacacagg cgagcagcca tggaaaggac gtcagcttcc
      241 ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa
      301 ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa
      361 ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc
      421 acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg
      481 atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca
      541 ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc
      601 tttcacaaat tttgtaatcc agaggttgat tgtcgagcgg ccgccactgt gctggatatc
      661 aaccactttg tacaagaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
      721 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
      781 actatggtcg acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga
      841 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctga
//
