LOCUS       sequence-63432-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral promoter-less Gateway destination vector, Zeo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X1 Zeo DEST (668-1)
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(128..252)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    283..871
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(336..356)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(754..765)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(922..942)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
       61 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
      121 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
      181 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
      241 gtacaaagtg gttgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac
      301 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
      361 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
      421 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
      481 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
      541 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
      601 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
      661 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
      721 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
      781 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
      841 agtcggatct ccctttgggc cgcctccccg cctggaattc tgcagatatc cggttagtaa
      901 tgagtttgga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
      961 ccccagcagg cagaagtatg caaagca
//
