LOCUS       sequence-63433-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral promoter-less Gateway destination vector, Zeo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X1 Zeo DEST (668-1)
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    318..434
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(701..745)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgttgatat ctgcagaatt
      301 ccaccacact ggacgataaa attttgaatt ttgtaatttg tttttgtaat tctttagttt
      361 gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc
      421 cttttctttt aaaagttaac cgataccgtc gagatccgtt cactaatcga atggatctgt
      481 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
      541 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
      601 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
      661 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
      721 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
      781 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
      841 aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat
      901 gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac
      961 agccaggatt cttgcctgga gctgcttgat gcccca
//
