LOCUS       sequence-63434-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral promoter-less Gateway destination vector, Zeo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X1 Zeo DEST (668-1)
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             95..328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(421..795)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(814..861)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(941..960)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttcctctca ctctctgata ttcatttctt tgcaagttat
       61 aaatactgaa taataagatg acatgaacta ctactgctag agattttcca cactgactaa
      121 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
      181 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
      241 tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag cagatcttgt
      301 cttcgttggg agtgagctag cccttccagt cccccctttt cttttaaaaa gtggctaaga
      361 tctacagctg ccttgtaagt cattggtctt aaaggtacca tttaaatctc gtagcacgtg
      421 tcagtcctgc tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa
      481 ctcccgcccc cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa
      541 gttcgtggac acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac
      601 ccaggccagg gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac
      661 gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg
      721 gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact
      781 ggtcaacttg gccatggttt agttcctcac cttgtcgtat tatactatgc cgatatacta
      841 tgccgatgat taattgtcaa cagggggagc tttttgcaaa agcctaggcc tccaaaaaag
      901 cctcctcact acttctggaa tagctcagag gcagaggcgg cctcggcctc tgcataaata
      961 aaaaaaatta gtcagccatg gggcgga
//
