LOCUS       sequence-63437-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of DsRed in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ
  TITLE     Global protein stability profiling in mammalian cells.
  JOURNAL   Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489.
  PUBMED    18988847
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-11-7- 7"; volume: "322"; issue: 
            "5903"; pages: "918-23"
COMMENT     Sequence Label: MSCV-CMV-DsRed-IRES-d24EGFP
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      186..195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(322..340)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(372..392)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     590..609
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     784..807
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgttcagat cgcctggaga cgccatccac gcttgttttt
       61 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      121 acggacctgc aggaattgat ccgcggccgc accggctagc gctaccggac tcagatccac
      181 cggtcgccac catggcctcc tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc
      241 gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      301 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
      361 cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg
      421 ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      481 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggct
      541 ccttcatcta caaggtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc
      601 agaagaagac tatgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
      661 tgaagggcga gatccacaag gccctgaagc tgaaggacgg cggccactac ctggtggagt
      721 tcaagtccat ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggact
      781 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggagcag tacgagcgcg
      841 ccgaggggcc gccaccacct gttcctgtag cggccaattc tgcagtcgac ggtaccgcgg
      901 gcccgggatc ggcccctctt ccttccc
//
