LOCUS       sequence-63438-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of DsRed in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ
  TITLE     Global protein stability profiling in mammalian cells.
  JOURNAL   Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489.
  PUBMED    18988847
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-11-7- 7"; volume: "322"; issue: 
            "5903"; pages: "918-23"
COMMENT     Sequence Label: MSCV-TRE-DsRed-IRES-d24EGFP
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..41
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      208..217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(344..362)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(394..414)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     612..631
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     806..829
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gaggctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
       61 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cccgaattcg
      121 agctcggtac ccggggatcc tctagtcagc tgacgcgtgc tagcgcggcc gcaccggcta
      181 gcgctaccgg actcagatcc accggtcgcc accatggcct cctccgagga cgtcatcaag
      241 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
      301 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      361 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
      421 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
      481 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      541 gactcctccc tgcaggacgg ctccttcatc tacaaggtga agttcatcgg cgtgaacttc
      601 ccctccgacg gccccgtaat gcagaagaag actatgggct gggaggcctc caccgagcgc
      661 ctgtaccccc gcgacggcgt gctgaagggc gagatccaca aggccctgaa gctgaaggac
      721 ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg
      781 cccggctact actacgtgga ctccaagctg gacatcacct cccacaacga ggactacacc
      841 atcgtg
//
