LOCUS       sequence-63441-e       10031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Barria A, Malinow R
  TITLE     Subunit-specific NMDA receptor trafficking to synapses.
  JOURNAL   Neuron. 2002 Jul 18;35(2):345-53.
  PUBMED    12160751
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2002-07-18"; volume: "35(2)"; pages: "345-53"
COMMENT     Sequence Label: pCI-EGFP-NR2a wt
FEATURES             Location/Qualifiers
     source          1..10031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1273..1275,1276..1986)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1273..1275,1276..1986)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1315..1336)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1576..1595)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1923..1944
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7145..7266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7156..7175)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7210..7229
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      7447..7902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7534..7553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7744..7765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7969..7988)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8088..8110
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8148..8166)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8234..8338
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8339..8407,8408..9199)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8557..8576)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9370..9958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9859..9878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagacgc gtagatctca cgtgagcatg
     1081 cgggaacccg ctaaacctgg tggctgctga ggcggccgag atgctcgtgc gcgcagcacg
     1141 ccccattgca tcctccacct tctccggcta cagggaccct aagtggcgac catgggcaga
     1201 ttgggctact ggaccttgct ggtattgccg gcccttctgg tctggcgcga tccggcgcag
     1261 aacgcggcta gggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1321 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1381 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1441 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1501 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1561 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1621 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1681 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1741 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1801 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1861 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1921 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1981 tacaaggcta gcgcggcgga gaagggtcct ccagcgctga acattgcggt gctgctgggt
     2041 cacagccacg acgtgacaga acgcgaactt cgaaatctgt ggggcccaga gcaggcaacc
     2101 ggcttgcccc tggatgtgaa cgtggtggcg ttattgatga accgcactga ccctaagagc
     2161 ctcatcacgc atgtgtgcga cctcatgtcc ggggcgcgca tccacggctt ggtgtttgga
     2221 gatgacacgg accaggaggc tgtggcccag atgctggatt ttatctcctc acagactttt
     2281 atccccatct tgggcattca tgggggtgca tctatgatca tggctgacaa ggatccgaca
     2341 tccacgttct tccagtttgg agcctccatc cagcagcaag ccacagttat gctgaagatc
     2401 atgcaggact acgactggca cgtcttctcc ctggtcacca ccatcttccc tggctaccga
     2461 gacttcatca gctttatcaa gacaacagtg gacaacagct ttgtgggctg ggatatgcag
     2521 aacgtgatca cactggacac ctccttcgag gacgccaaga cgcaagtcca gctgaagaag
     2581 atccattctt ctgtcatcct gctctactgc tccaaagacg aggctgtcct catcctgagc
     2641 gaggctcgct ccttcggcct cactggctat gatttcttct ggattgtccc cagtttggtg
     2701 tctgggaaca cagagctcat ccccaaagag tttccatcag gtctcatttc agtctcttat
     2761 gacgactggg actacagcct ggaggcaaga gtgagagacg gtcttgggat cttaaccact
     2821 gccgcatcct ccatgttgga gaagttctcc tacattcctg aggccaaggc cagctgctat
     2881 gggcaggcag agaagccaga gaccccgcta cacaccctgc accaattcat ggtcaatgtg
     2941 acttgggatg gcaaggactt gtccttcact gaggaaggtt accaggtgca ccccaggctt
     3001 gtggtgatcg tgctgaacaa ggaccgggag tgggaaaagg tgggcaagtg ggagaaccag
     3061 acgctgagcc tgaggcacgc tgtgtggcca aggtacaagt ccttttctga ctgcgagcca
     3121 gatgacaacc acctcagcat tgtcaccttg gaggaagccc ccttcgtcat cgtagaggac
     3181 atagaccccc tgactgagac ctgtgtgagg aacacggtgc cctgtcggaa gttcgtcaag
     3241 atcaacaatt caaccaacga agggatgaat gtgaagaaat gctgcaaggg gttctgcatc
     3301 gacatcctca agaagctgtc cagaactgtg aagttcacct atgacctcta cctggtgacc
     3361 aatgggaagc atgggaaaaa ggttaacaat gtgtggaatg gaatgatagg tgaagtggtc
     3421 tatcaacgag cagtcatggc tgtgggctca ctcaccatca atgaggagcg ttcggaagtg
     3481 gtggacttct cggtgccctt tgtggagaca ggaatcagcg tcatggtctc caggagtaat
     3541 ggcactgtct ccccttctgc tttcctcgaa cccttcagtg cctccgtctg ggtgatgatg
     3601 tttgtgatgc tgctcatcgt ctcagccatt gctgtcttcg tttttgaata cttcagtcct
     3661 gttggataca acagaaactt agccaaaggg aaagctcccc acgggccttc ttttactatt
     3721 ggaaaagcta tatggctcct ctggggcctg gtcttcaaca attctgtgcc tgtccagaat
     3781 cctaaaggca caaccagcaa gatcatggtg tcagtgtggg ccttctttgc tgtcatcttc
     3841 ctggccagtt acacagccaa cctggctgcc ttcatgatcc aggaggagtt tgtggaccaa
     3901 gtgactggcc tcagtgacaa gaagttccag agacctcatg actattctcc acctttccga
     3961 tttgggacgg tacccaatgg aagtacagag aggaatattc gtaacaacta cccgtatatg
     4021 caccagtaca tgaccagatt caaccagagg ggagtggagg atgccttggt cagcttgaaa
     4081 accgggaagt tggacgcttt catctatgac gcagccgtct tgaactacaa ggccgggagg
     4141 gatgaaggct gtaaactggt gaccattggg agcgggtaca tctttgctac cacaggctat
     4201 ggaattgcgc tgcagaaggg ctcaccctgg aagaggcaga ttgacctcgc tctgctccag
     4261 tttgttggtg atggtgagat ggaggagctg gagacactgt ggcttacggg catctgccac
     4321 aacgagaaga atgaggtgat gagtagccag ctggacatcg ataacatggc gggcgtgttc
     4381 tacatgctgg ctgcagccat ggccctcagc ctcatcacct tcatctggga gcacctcttc
     4441 tactggaagc tgcgcttctg cttcacaggc gtgtgctctg accggcccgg gctgctcttc
     4501 tccatcagca ggggcatcta tagttgcatc catggggtac acattgaaga aaagaagaaa
     4561 tctccagatt tcaatctgac tggatcacag agcaacatgc taaagcttct tcggtcagct
     4621 aaaaacatct ccaatatgtc caacatgaac tcctcaagaa tggactcacc taaaagagct
     4681 actgacttca ttcaaagagg gtcacttatt gtggacatgg tttcagacaa gggaaatttg
     4741 atatattcag acaacagatc ctttcaaggg aaggacagta tatttggaga caacatgaat
     4801 gaactccaaa catttgtggc caacaggcac aaggataatc tcagtaacta tgtgtttcaa
     4861 ggacagcatc ctctcactct caatgagtcc aaccctaaca cagtagaggt ggctgtcagc
     4921 actgaatcca aagggaactc ccgaccccgg cagctttgga agaaatccat ggagtctcta
     4981 cgccaggatt ctctaaacca gaacccagtc tcccagaggg atgagaagac tgcagagaat
     5041 cggacccact cgctaaagag tcctaggtat cttccagaag aggtagccca ctctgacatt
     5101 tcagaaacct caagccgggc cacatgccac agggagccag ataacaataa gaaccacaag
     5161 accaaggata acttcaaacg gtcaatggcc tctaagtatc ccaaggactg tagcgatgtt
     5221 gaccgcacct acatgaaaac caaagcaagt tctcccaggg ataagatcta taccattgat
     5281 ggtgagaagg agcccagctt ccacttagat cctcctcagt ttgttgagaa tataaccctg
     5341 cctgagaatg tgggcttccc agatacctac caagatcaca atgagaactt ccgcaagggg
     5401 gactccacac tgcccatgaa caggaaccca ttacataatg aagacgggct tcccaacaat
     5461 gaccaatata aactctatgc caagcacttt accttgaaag acaagggttc cccacacagt
     5521 gagggcagtg atcgataccg gcagaactcc acacattgca gaagctgcct ttcgaatctg
     5581 cccacctact caggccactt taccatgagg tctcctttca agtgtgatgc ctgtctgcgg
     5641 atggggaatc tctatgacat tgatgaagac cagatgcttc aggagacagg taacccagct
     5701 actcgggagg aggtctacca gcaggactgg tcacagaaca acgccctcca gttccagaag
     5761 aacaagctaa ggattaaccg acagcactcc tatgataaca ttctggacaa acccagagag
     5821 atagacctta gcaggccctc ccggagcata agcctcaagg acagggaacg gctactggag
     5881 ggcaacttgt atgggagcct gttcagtgtc ccctcaagca aactcttggg gaacaaaagc
     5941 tcccttttcc cccaaggtct ggaggacagc aagaggagca agtctctctt gccagaccac
     6001 gcctccgata atcctttcct ccacacgtat ggggatgacc aacgcttagt tatcgggaga
     6061 tgtccctcgg acccttacaa acactcattg ccatcacagg cggtaaatga cagctatctt
     6121 cggtcatcct tgaggtcaac agcatcatat tgctccaggg acagtcgggg ccacagtgat
     6181 gtgtatattt cagagcatgt tatgccttat gctgcaaata agaataccat gtactctacc
     6241 cccagggttt taaattcctg cagcaataga cgagtgtaca agaaaatgcc tagtatcgaa
     6301 tctgatgttt aagatcttcc atcagtattt atctataagg aaacatatag aatggccaac
     6361 attatagagg gtaaatgttg gatgtccgat agcaccctac taggaggaag agggtacagg
     6421 gaggtacttt ttgttggctc ttttgcacat ggctccatgc cataatcttc cactcaagga
     6481 atcttgtgag atatgtgctg agcacagcat ataccacgta ggtgaatcct taaccaaaaa
     6541 caaataaata cacatgggca agtctcccag acatggcgac tgggcacggc ggcaataatg
     6601 gtgcatcaga cggcgatggt gacattgtgg ttgcctatat tccaaattcc atgaagatca
     6661 atccaccacg taacttcccc catcagaaat gcctcacagt ttctctaata cagaataagc
     6721 aatatggtat gcatgtaagt ctgacgcaga caataagaca gttaagaatg catctgcact
     6781 gtagtaagat tgacatgtgc aaggattaag aagtttggct tgtaacagtt ctcagatttc
     6841 ttgttatgcc ttccatgaca accctggatc agtccccctt aaactccagg ttcgcccccc
     6901 cccccaaaga atagcaaatc agcgtgtttg tggcacccac actactttca ttgtaggctt
     6961 cccccaagac acacctggaa caaatagccc cagggactct caggtggaga actgtaagaa
     7021 tctctttgga tgttaatgtg tgtgtatctc tcaccctcag cttcattggt cttgttcgga
     7081 gctgcataaa cacttttatg tcttcgatga cctcgacccg ggcggccgct tcgagcagac
     7141 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     7201 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     7261 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     7321 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgggctgg
     7381 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7441 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7501 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7561 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7621 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7681 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7741 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7801 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7861 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     7921 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     7981 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     8041 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     8101 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     8161 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     8221 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8281 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8341 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8401 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8461 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8521 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8581 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8641 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8701 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8761 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8821 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8881 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     8941 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9001 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9061 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9121 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9181 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9241 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9301 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9361 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9421 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9481 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9541 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9601 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9661 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     9721 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     9781 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     9841 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     9901 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9961 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc
    10021 tcgacagatc t
//
