LOCUS       sequence-63467-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Cheutin T, McNairn AJ, Jenuwein T, Gilbert DM, Singh PB, Misteli T
  TITLE     Maintenance of stable heterochromatin domains by dynamic HP1 
            binding.
  JOURNAL   Science. 2003 Jan 31. 299(5607):721-5.
  PUBMED    12560555
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2003 Jan 31. 299(5607):721-5."
COMMENT     Sequence Label: GFP-HP1alpha
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    611..745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(648..667)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     702..721
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(781..799)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(820..836)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(820..836)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(833..855)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    844..860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(868..874,875..892,893..898))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tggcatggat gaactataca agggatccgg aaagaaaacc aagcggacag ctgacagttc
       61 ttcttcagag gatgaggagg agtatgtcgt ggagaaggtg ctagacaggc gcgtggttaa
      121 gggacaagtg gaatatctac tgaagtggaa aggcttttct gaggagcaca atacttggga
      181 acctgagaaa aacttggatt gccctgagct aatttctgaa tttatgaaaa agtataagaa
      241 gatgaaggag ggtgaaaata ataaacccag ggagaagtca gaaagtaaca agaggaaatc
      301 caatttctca aacagtgccg atgacatcaa atctaaaaaa aagagagagc agagcaatga
      361 tatcgctcgg ggctttgaga gaggactgga accagaaaag atcattgggg caacagattc
      421 ctgtggtgat ttaatgttcc taatgaaatg gaaagacaca gatgaagctg acctggttct
      481 tgcaaaagaa gctaatgtga aatgtccaca aattgtgata gcattttatg aagagagact
      541 gacatggcat gcatatcctg aggatgcgga aaacaaagag aaagaaacag caaagagcta
      601 agcttaaccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      661 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      721 caatgtatct tatcatgtct ggatcgacct cgaggggggg cccggtacca gcttttgttc
      781 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      841 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      901 ctggggtgcc taatgagtga
//
