LOCUS       sequence-63469-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Cheutin T, McNairn AJ, Jenuwein T, Gilbert DM, Singh PB, Misteli T
  TITLE     Maintenance of stable heterochromatin domains by dynamic HP1 
            binding.
  JOURNAL   Science. 2003 Jan 31. 299(5607):721-5.
  PUBMED    12560555
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2003 Jan 31. 299(5607):721-5."
COMMENT     Sequence Label: GFP-HP1alpha
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        247..265
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     247..264
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     278..297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      350..359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             362..379
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(410..438)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
      121 tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc
      181 cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca
      241 catacgattt aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc
      301 cactcccagg tccaactgca cctcggttct gggccgctct agaactagcg ccaccatggg
      361 acatcaccat caccatcaca tgagtaaagg agaagaactt ttcactggag ttgtcccaat
      421 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      481 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      541 tgttccatgg ccaacacttg tcactacttt cacttatggt gttcaatgct tttcaagata
      601 cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      661 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      721 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      781 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      841 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      901 aagcgttcaa ctagca
//
