LOCUS       sequence-63495-e        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALETF_v2 (NI)
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        join(850..855,856..873,874..880)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(1000..1019)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg gcgtacggcc accatgtcgc ggacccggct cccttcccca
      121 cccgcaccca gcccagcgtt ttcggccgac tcgttctcag acctgcttag gcagttcgac
      181 ccctcactgt ttaacacatc gttgttcgac tcccttcctc cgtttggggc gcaccatacg
      241 gaggcggcca ccggggagtg ggatgaggtg cagtcgggat tgagagctgc ggatgcacca
      301 cccccaacca tgcgggtggc cgtcaccgct gcccgaccgc cgagggcgaa gcccgcacca
      361 aggcggaggg cagcgcaacc gtccgacgca agccccgcag cgcaagtaga tttgagaact
      421 ttgggatatt cacagcagca gcaggaaaag atcaagccca aagtgaggtc gacagtcgcg
      481 cagcatcacg aagcgctggt gggtcatggg tttacacatg cccacatcgt agccttgtcg
      541 cagcaccctg cagcccttgg cacggtcgcc gtcaagtacc aggacatgat tgcggcgttg
      601 ccggaagcca cacatgaggc gatcgtcggt gtggggaaac agtggagcgg agcccgagcg
      661 cttgaggccc tgttgacggt cgcgggagag ctgagagggc ctccccttca gctggacacg
      721 ggccagttgc tgaagatcgc gaagcgggga ggagtcacgg cggtcgaggc ggtgcacgcg
      781 tggcgcaatg cgctcacggg agcacccctc aacctgacag agaccgcggc cgcattaggc
      841 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
      901 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      961 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgc
//
