LOCUS       sequence-63496-e        1192 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, 
            Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel 
            AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P,
            Mesirov JP, Beroukhim R, Hahn WC
  TITLE     Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer
            drivers.
  JOURNAL   Cancer Discov. 2013 Jun 13. [Epub ahead of print]
  PUBMED    23764425
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2013 Jun 13. [Epub ahead of print]"
COMMENT     Sequence Label: pWzl_neo_DEST_flag_hcRED
FEATURES             Location/Qualifiers
     source          1..1192
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..32
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    39..63
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             66..749
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(751..775)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(890..907)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ccnccntgga ctacaaagac gatgacgaca agcaattgac aagtttgtac aaaaaagttg
       61 gcaccatggt gagcggcctg ctgaaggaga gtatgcgcat caagatgtac atggagggca
      121 ccgtgaacgg ccactacttc aagtgcgagg gcgagggcga cggcaacccc ttcgccggca
      181 cccagagcat gagaatccac gtgaccgagg gcgcccccct gcccttcgcc ttcgacatcc
      241 tggccccctg ctgcgagtac ggcagcagga ccttcgtgca ccacaccgcc gagatccccg
      301 acttcttcaa gcagagcttc cccgagggct tcacctggga gagaaccacc acctacgagg
      361 acggcggcat cctgaccgcc caccaggaca ccagcctgga gggcaactgc ctgatctaca
      421 aggtgaaggt gcacggcacc aacttccccg ccgacggccc cgtgatgaag aacaagagcg
      481 gcggctggga gcccagcacc gaggtggtgt accccgagaa cggcgtgctg tgcggccgga
      541 acgtgatggc cctgaaggtg ggcgaccggc acctgatctg ccaccactac accagctacc
      601 ggagcaagaa ggccgtgcgc gccctgacca tgcccggctt ccacttcacc gacatccggc
      661 tccagatgct gcggaagaag aaggacgagt acttcgagct gtacgaggcc agcgtggccc
      721 ggtacagcga cctgcccgag aaggccaact tgccaacttt cttgtacaaa gtggtttgat
      781 ctagtcgacc aattccggtt attttccacc atattgccgt cttttggcaa tgtgagggcc
      841 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
      901 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
      961 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg ngacaggtgc
     1021 ctctgnnnna aaagccacnn gtatnanann acacctgcaa aggngnacna ccccnnnncc
     1081 acntnnngnn nnnnnannnn ngaangnant caaatnnnnn nnncagcgta tcnannnggg
     1141 nnnaagnatn ccnnaannnc ccnnnnnatg gnntnnnatc ngngnncntc gg
//
