LOCUS       sequence-63510-e        2730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Bryan A, Swanson MS
  TITLE     Oligonucleotides stimulate genomic alterations of Legionella 
            pneumophila.
  JOURNAL   Mol Microbiol. 2011 Apr;80(1):231-47. doi: 
            10.1111/j.1365-2958.2011.07573.x. Epub 2011 Feb 24.
  PUBMED    21306445
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2011.07573.x"; journalName: "Mol Microbiol"; 
            date: "2011-04"; volume: "80"; issue: "1"; pages: "231-47"
COMMENT     Sequence Label: pR6Kcat-rdxA-rpsL
FEATURES             Location/Qualifiers
     source          1..2730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        145..247
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(314..333)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2332..2720
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 cgcggccgca ctagtacctg gtggaggata ttcatatgga ccatggaatt ctgtgacgga
       61 agatcacttc gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc
      121 caacttttgg cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa
      181 tgaaataaga tcactaccgg gcgtattttt tgagttgtcg agattttcag gagctaagga
      241 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
      301 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
      361 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
      421 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattacgta tggcaatgaa
      481 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
      541 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
      601 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
      661 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
      721 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
      781 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
      841 cttccatgtc ggcagatgct taatgaatac aacagtactg cgatgagtgg cagggcaatt
      901 tgagcatggg gcagatttta agcttattta tggtaattgt ttcgttaggg attttattgt
      961 atgctacaaa aaattctaaa aaaataaagg aaaatcaatg aaatttttgg atcaagaaaa
     1021 aagaagacaa ttattaaacg agcgccattc ttgcaagatg tttgatagcc attatgagtt
     1081 ttctagcaca gaattagaag aaatcgctga aatcgccagg ctatcgccaa gctcttacaa
     1141 cacgcagcca tggcattttg tgatggttac tgataaggat ttaaaaaaac aaattgcagc
     1201 gcacagctat ttcaatgaag agatgattaa aagcgcttca gcgttaatgg tggtatgctc
     1261 tttaagaccc agcgagttgt taccacacgg ccactacatg caaaatctct atccggagtc
     1321 ttataaagtt agagtgatcc cctcttttgc tcaaatgctt ggcgtgagat tcaaccacag
     1381 catgcaaaga ttagaaagct atattttaga gcaatgctat atcgctgtgg ggcaaatttg
     1441 catgggcgtg agcttaatgg gattggatag ttgcattatt ggaggctttg atcctttaaa
     1501 ggtgggcgaa gttttagaag agcgtatcaa taagcctaaa atcgcatgct tgatcgcttt
     1561 gggcaagagg gtggcagaag cgagtcaaaa atcaagaaaa tcaaaagttg atgcgattac
     1621 ttggttgtga cgcggtttga ctggtcaaat ttcgaacgac atcattctca ttgactatac
     1681 tgatttcgtc agacttacgg ttaagcaccc cagccagatg gcctggtgat ggcgggatcg
     1741 ttgtatattt cttgacacct tttcggcatc gccctaaaat tcggcgtcct catattgtgt
     1801 gaggacgttt tattacgtgt ttacgaagca aaagctaaaa ccaggagcta tttaatggca
     1861 acagttaacc agctggtacg caaaccacgt gctcgcaaag ttgcgaaaag caacgtgcct
     1921 gcgctggaag catgcccgca aaaacgtggc gtatgtactc gtgtatatac taccactcct
     1981 aaaaaaccga actccgcgct gcgtaaagta tgccgtgttc gtctgactaa cggtttcgaa
     2041 gtgacttcct acatcggtgg tgaaggtcac aacctgcagg agcactccgt gatcctgatc
     2101 cgtggcggtc gtgttaaaga cctcccgggt gttcgttacc acaccgtacg tggtgcgctt
     2161 gactgctccg gcgttaaaga ccgtaagcag gctcgttcca agtatggcgt gaagcgtcct
     2221 aaggcttaag aattcgcggc cgcatcgatg gatccgtact gcgatgagtg gcagggctca
     2281 tttttaattt ttgcggccgc aagatccggc cacgatgcgt ccggcgcaga ggatctgaag
     2341 atcagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg tactaagctc
     2401 tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg ctaacgtact
     2461 aagctctcat ggctaacgta ctaagctctc atgtttcacg tactaagctc tcatgtttga
     2521 acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa gttttataag
     2581 aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt ggacaacaag
     2641 ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt gagtgacaca
     2701 ggaacactta acggctgaca tgggaattag
//
