LOCUS       sequence-63516-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Cheeseman IM, Desai A
  TITLE     A combined approach for the localization and tandem affinity 
            purification of protein complexes from metazoans.
  JOURNAL   Sci STKE. 2005 Jan 11;2005(266):pl1.
  PUBMED    15644491
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci STKE.";
            date: "2005-01-11"; volume: "2005(266)"; pages: "pl1"
COMMENT     Sequence Label: pIC112
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..218
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             219..242
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(397..415)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     665..684
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     859..882
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      121 ggtggaattc tgcagatatc cagcacagtg gcggccgcgg cagcagccat catcatcatc
      181 atcacagcag cagcctggaa gttctgttcc aggggcccct ggaagttctg ttccaggggc
      241 cctccggagg gatggtgagc aagggcgagg agaataacat ggccgtcatc aaggagttca
      301 tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      361 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      421 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      481 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
      541 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      601 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      661 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
      721 ccgaggacgg cgccctgaag ggcgagatca agatgaggct gaagctgaag gacggcggcc
      781 actacgacgc cgaagtcaag accacctaca tggccaagaa gcccgtgcag ctgcccggcg
      841 cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac accatcgtgg
      901 aacagtacga
//
