LOCUS       sequence-63517-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_159_Venus_229
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..42
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        58..167
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             188..205
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             215..244
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atacgactca ctatagggga attgtgagcg gataacaatt ccccggagtt aatccgggac
       61 ctttaattca acccaacaca atatattata gttaaataag aattattatc aaatcatttg
      121 tatattaatt aaaatactat actgtaaatt acattttatt tacaatcaaa ggagatatac
      181 catggcacac catcaccacc accacgggag cggggagcag aaactaatat cagaggagga
      241 tcttggctca ggttcaggat ccgacggcgt gctgaagggc gacgtcaagc acaagctgct
      301 gctggagggc ggcggccacc accgcgttga cttcaagacc atctacaggg ccaagaaggc
      361 ggtgaagctg cccgactatc actttgtgga ccaccgcatc gagatcctga accacgacaa
      421 ggactacaac aaggtgaccg tttacgagag cgccgtggcc cgcaactcca ccgacggcat
      481 ggacgagctg tacaagggtg ctagcggagg aatggtgagc aagggcgagg agaccacaat
      541 gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg gagggcaacg tgaatggcca
      601 cgccttcgtg atcgagggcg agggcgaggg caagccctac gacggcacca acaccatcaa
      661 cctggaggtg aaggagggag cccccctgcc cttctcctac gacattctga ccaccgcgtt
      721 cgcctacggc aacagggcct tcaccaagta ccccgacgac atccccaact acttcaagca
      781 gtccttcccc gagggctact cttgggagcg caccatgacc ttcgaggaca aggg
//
