LOCUS       sequence-63536-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Ye B, Kim JH, Yang L, McLachlan I, Younger S, Jan LY, Jan YN
  TITLE     Differential regulation of dendritic and axonal development by the 
            novel Kruppel-like factor Dar1.
  JOURNAL   J Neurosci. 2011 Mar 2;31(9):3309-19. doi: 
            10.1523/JNEUROSCI.6307-10.2011.
  PUBMED    21368042
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.6307-10"; journalName: "J Neurosci"; date: 
            "2011-03-2- 2"; volume: "31"; issue: "9"; pages: "3309-19"
COMMENT     Sequence Label: UAS-dar1-V5
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(280..300)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          430..495
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(585..602)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(612..653)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt
      121 caaggcacta tacatcaaat atttttccat aattttcttg tatagcagtg cagctttttc
      181 ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa
      241 aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg
      301 aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga
      361 gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat cagttccata
      421 ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt atacacttaa
      481 aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac
      541 accacagaag taaggttcct tcacaaagat cctctagaaa ctcaatggtg atggtgatga
      601 tgaccggtac gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaag
      661 ggccctctag atttattctt gggcaagtgg cgcttcatgt ggagcgccag gtgatcactc
      721 ctcgcaaagc tcctctcgca cacgatgcat ttgaagggtt tggcccccgt gtgcttccgg
      781 tagtgcctcg ttagctcgtc cgacctggcg aatctccact cgcattccgg ccactggcag
      841 gtgtatggct tctca
//
