LOCUS       sequence-63539-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pCambia1300-PacI
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(165..841)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(208..228)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
       61 ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccggatctg
      121 cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc
      181 aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg
      241 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
      301 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
      361 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
      421 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
      481 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ctctgagact
      541 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc
      601 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      661 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
      721 gcaatgatgg catttgtagg tgccaccttc cctttctact gtccttttga tgaagtgaca
      781 gatagctggg caatggaatc cgaggaggtt tcccgacatt accctttgtt gaaaagtctc
      841 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
      901 gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt
      961 tggccgattc attaatg
//
