LOCUS       sequence-63545-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu
            K, Bokoch GM, Hahn KM
  TITLE     Rho family proteins modulate rapid apoptosis induced by cytotoxic T 
            lymphocytes and Fas.
  JOURNAL   J Biol Chem. 2000 Mar 31. 275(13):9725-33.
  PUBMED    10734125
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Mar 31. 275(13):9725-33."
COMMENT     Sequence Label: pcDNA3-EGFP-Rac1-Q61L
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(9..188,189..191,192..587)
                     /codon_start=1
                     /product="constitutively active mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (Q61L)
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGLEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     promoter        complement(615..633)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(616..633)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(653..670)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    659..883
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aggaattcat gcaggccatc aagtgtgtgg tggtgggaga cggagctgta ggtaaaactt
       61 gcctactgat cagttacaca accaatgcat ttcctggaga atatatccct actgtctttg
      121 acaattattc tgccaatgtt atggtagatg gaaaaccggt gaatctgggc ttatgggata
      181 cagctggact agaagattat gacagattac gccccctatc ctatccgcaa acagatgtgt
      241 tcttaatttg cttttccctt gtgagtcctg catcatttga aaatgtccgt gcaaagtggt
      301 atcctgaggt gcggcaccac tgtcccaaca ctcccatcat cctagtggga actaaacttg
      361 atcttaggga tgataaagac acgatcgaga aactgaagga gaagaagctg actcccatca
      421 cctatccgca gggtctagcc atggctaagg agattggtgc tgtaaaatac ctggagtgct
      481 cggcgctcac acagcgaggc ctcaagacag tgtttgacga agcgatccga gcagtcctct
      541 gcccgcctcc cgtgaagaag aggaagagaa aatgcctgct gttgtaactc gagcatgcat
      601 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
      661 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
      721 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
      781 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
      841 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga gg
//
