LOCUS       sequence-63546-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Bonnet J, Yin P, Ortiz ME, Subsoontorn P, Endy D
  TITLE     Amplifying genetic logic gates.
  JOURNAL   Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758.
            Epub 2013 Mar 28.
  PUBMED    23539178
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232758"; journalName: "Science"; date: "2013-05-3-
            3"; volume: "340"; issue: "6132"; pages: "599-603"
COMMENT     Sequence Label: AND-gate-V2.0
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             626..634
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_RNA        complement(728..777)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    784..831
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 gaacgctttg cgtgctcaga tagtgattgt ctggcagcag aacaggacca tcaccgattg
       61 gagtgttttg ctggtagtga tcagccagct gcacgctgcc atcctccacg ttgtggcgaa
      121 ttttaaaatt cgctttagtg ccattttttt gtttatcggc ggtgatgtaa acattgtggc
      181 tgttaaaatt gtattccagc ttatggccca ggatattgcc gtcttcttta aagtcaatgc
      241 ctttcagctc aatgcggttt accagagtat cgccttcaaa tttcacttcc gcacgcgttt
      301 tgtacgtgcc gtcatcctta aaggaaatcg tgcgttcctg cacatagcct tccggcatgg
      361 cggacttgaa gaagtcatgc tgcttcatat ggtccggata acgagcaaag cactgaacac
      421 cataagtcag cgtcgttacc agagtcggcc aaggtaccgg cagtttacca gtagtacaga
      481 tgaacttcag cgtcagttta ccattagttg cgtcaccttc accctcgcca cgcacggaaa
      541 acttatgacc gttgacatca ccatccagtt ccaccagaat agggacgaca ccagtgaaca
      601 gctcttcgcc tttacgcatt agaaaacctc cttagcatga ttaagatgtt tcagtacgaa
      661 aattgctttc attgttgatc tcctttttaa gtgaacttgg gcccttaaac aaaattattt
      721 gtagaggctg tttcgtcctc acggactcat cagaccggaa agcacatccg gtgacagctt
      781 cgtggtttgt ctggtcaacc accgcggtct cagtggtgta cggtacaaac cctttctttt
      841 gggtatagcg tc
//
