LOCUS       sequence-63547-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with mKO1 with CaUra3 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-mKO1-CaUra3
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..687
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
     primer_bind     complement(763..787)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cggatcggtg acggtgctgg tttaattaac atggtgagtg tgattaaacc agagatgaag
       61 atgaggtact acatggacgg ctccgtcaat gggcatgagt tcacaattga aggtgaaggc
      121 acaggcagac cttacgaggg acatcaagag atgacactac gcgtcacaat ggccaagggc
      181 gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt tctgttacgg ccacagacct
      241 tttactaaat atccagaaga gataccagac tatttcaaac aagcatttcc tgaaggcctg
      301 tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg cttcagtcag tgcgcatata
      361 agccttagag gaaacacctt ctaccacaaa tccaaattta ctggggttaa ctttcctgcc
      421 gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc catcaaccga gaaaattact
      481 gccagcgacg gagttctgaa gggtgatgtt acgatgtacc taaaacttga aggaggcggc
      541 aatcacaaat gccaattcaa gactacttac aaggcggcaa aaaagattct taaaatgcca
      601 ggaagccatt acatcagcca tcgcctcgtc aggaaaaccg aaggcaacat tactgagctg
      661 gtagaagatg cagtagctca ttcctgaggc gcgccacttc taaataagcg aatttcttat
      721 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
      781 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactcttt
//
