LOCUS       sequence-63548-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with mDsRed with SpHis5 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-mDsRed-SpHis5
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     429..448
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cggatcggtg acggtgctgg tttaattaac atggacaaca ccgaggacgt catcaaggag
       61 ttcatgcagt tcaaggtgcg catggagggc tccgtgaacg gccactactt cgagatcgag
      121 ggcgagggcg agggcaagcc ctacgagggc acccagaccg ccaagctgca ggtgaccaag
      181 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag
      241 gcctacgtga agcaccccgc cgacatcccc gactacatga agctgtcctt ccccgagggc
      301 ttcacctggg agcgctccat gaacttcgag gacggcggcg tggtggaggt gcagcaggac
      361 tcctccctgc aggacggcac cttcatctac aaggtgaagt tcaagggcgt gaacttcccc
      421 gccgacggcc ccgtaatgca gaagaagact gccggctggg agccctccac cgagaagctg
      481 tacccccagg acggcgtgct gaagggcgag atctcccacg ccctgaagct gaaggacggc
      541 ggccactaca cctgcgactt caagaccgtg tacaaggcca agaagcccgt gcagctgccc
      601 ggcaaccact acgtggactc caagctggac atcaccaacc acaacgagga ctacaccgtg
      661 gtggagcagt acgagcacgc cgaggcccgc cactccggct cccagtaggg cgcgccactt
      721 ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaa
//
