LOCUS       sequence-63553-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with mKO1 with SpHis5 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-mKO1-SpHis5
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..676
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 cggtgctggt ttaattaaca tggtgagtgt gattaaacca gagatgaaga tgaggtacta
       61 catggacggc tccgtcaatg ggcatgagtt cacaattgaa ggtgaaggca caggcagacc
      121 ttacgaggga catcaagaga tgacactacg cgtcacaatg gccaagggcg ggccaatgcc
      181 tttcgcgttt gacttagtgt cacacgtgtt ctgttacggc cacagacctt ttactaaata
      241 tccagaagag ataccagact atttcaaaca agcatttcct gaaggcctgt catgggaaag
      301 gtcgttggag ttcgaagatg gtgggtccgc ttcagtcagt gcgcatataa gccttagagg
      361 aaacaccttc taccacaaat ccaaatttac tggggttaac tttcctgccg atggtcctat
      421 catgcaaaac caaagtgttg attgggagcc atcaaccgag aaaattactg ccagcgacgg
      481 agttctgaag ggtgatgtta cgatgtacct aaaacttgaa ggaggcggca atcacaaatg
      541 ccaattcaag actacttaca aggcggcaaa aaagattctt aaaatgccag gaagccatta
      601 catcagccat cgcctcgtca ggaaaaccga aggcaacatt actgagctgg tagaagatgc
      661 agtagctcat tcctgaggcg cgccacttct aaataagcga atttcttatg atttatgatt
      721 tttattatta aataagttat aaaaaaaaa
//
