LOCUS       sequence-63563-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALETF_v2 (HD)
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             734..754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             779..928
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
       61 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
      121 tcgacggtct cgactcacgc ctgagcaggt agtggctatt gcatcccatg acgggggcag
      181 acccgcactg gagtcaatcg tggcccagct ttcgaggccg gaccccgcgc tggccgcact
      241 cactaatgat catcttgtag cgctggcctg cctcggcgga cgacccgcct tggatgcggt
      301 gaagaagggg ctcccgcacg cgcctgcatt gattaagcgg accaacagaa ggattcccga
      361 gaggacatca catcgagtgg cagatcacgc gcaagtggtc cgcgtgctcg gattcttcca
      421 gtgtcactcc caccccgcac aagcgttcga tgacgccatg actcaatttg gtatgtcgag
      481 acacggactg ctgcagctct ttcgtagagt cggtgtcaca gaactcgagg cccgctcggg
      541 cacactgcct cccgcctccc agcggtggga caggattctc caagcgagcg gtatgaaacg
      601 cgcgaagcct tcacctacgt caactcagac acctgaccag gcgagccttc atgcgttcgc
      661 agactcgctg gagagggatt tggacgcgcc ctcgcccatg catgaagggg accaaactcg
      721 cgcgtcagct agccccaaga agaagagaaa ggtggaggcc agcggttccg gacgggctga
      781 cgcattggac gattttgatc tggatatgct gggaagtgac gccctcgatg attttgacct
      841 tgacatgctt ggttcggatg cccttgatga ctttgacctc gacatgctcg gcagtgacgc
      901 ccttgatgat ttcgacctgg acatgctgat taactctaga ggcagtggag agggcaga
//
