LOCUS       sequence-63573-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     Plasmids to assay for nuclease cleavage in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHEX2 T494 TALENs
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        42..60
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     42..59
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(101..808)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 ccggccgcca tggccgcggg atatcactag tgcggccgca gatttaggtg acactataga
       61 atatgcatca ctagtaagct agcttgcatg cctgcaggtc ctgctgagcc tcgacatgtt
      121 gtcgcaaaat tcgccctgga cccgcccaac gatttgtcgt cactgtcaag gtttgacctg
      181 cacttcattt ggggcccaca tacaccaaaa aaatgctgca taattctcgg ggcagcaagt
      241 cggttacccg gccgccgtgc tggaccgggt tgaatggtgc ccgtaacttt cggtagagcg
      301 gacggccaat actcaacttc aaggaatctc acccatgcgc gccggcgggg aaccggagtt
      361 cccttcagtg agcgttatta gttcgccgct cggtgtgtcg tagatactag cccctggggc
      421 acttttgaaa tttgaataag atttatgtaa tcagtctttt aggtttgacc ggttctgccg
      481 ctttttttaa aattggattt gtaataataa aacgcaattg tttgttattg tggcgctcta
      541 tcatagatgt cgctataaac ctattcagca caatatattg ttttcatttt aatattgtac
      601 atataagtag tagggtacaa tcagtaaatt gaacggagaa tattattcat aaaaatacga
      661 tagtaacggg tgatatattc attagaatga accgaaaccg gcggtaagga tctgagctac
      721 acatgctcag gttttttaca acgtgcacaa cagaattgaa agcaaatatc atgcgatcat
      781 aggcgtctcg catatctcat taaagcagga ctctagagga tccaagctta tcgatttc
//
