LOCUS       sequence-63575-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  TITLE     Plasmids to assay for nuclease cleavage in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGreenII 0000 (No LUC)
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    26..133
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     42..58
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     43..59
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(93..109)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(93..109)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(146..166)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(146..164)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(185..201)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(185..201)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(198..220)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    209..225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(233..239,240..257,258..263))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    278..299
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(416..433)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    538..562
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     complement(733..752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga
       61 taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag cggccgccac
      121 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
      181 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      241 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      301 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      361 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      421 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      481 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg aaggccttga
      541 caggatatat tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat ctcatgtgag
      601 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      661 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      721 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      781 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      841 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      901 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatc
//
